Category, Microbiology Laboratory. Test, Test Method Description. Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. TEST METHODS ARE DELIVERED AS A LINK IN YOUR EMAILED RECEIPT. AATCC TM, Antibacterial Activity Assessment of Textile Materials. Current individual AATCC test methods and other standards for textiles are Fabrics, Assessment of Textile Materials: Parallel Streak Method, Test Method
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Until a precision statement is generated for this test method, use standard statistical techniques in mtehod any comparisons of test results for either within-laboratory or between-laboratory averages.
Specimens of the test material, including corresponding untreated controls of the same material, are placed in intimate contact with growth agar which has been previously streaked with test organism.
Foreword The Parallel Streak Method has filled a need for a relatively quick and easily executed qualitative method to determine antibacterial activity of diffusable antimicrobial agents on treated textile materials.
Peptone Bacto-peptone see Uses and Limitations 1.
Adjust to pH 6. Users of this test method are expressly advised that determination of the validity of any such patent rights, and the risk of infringement of such rights, are entirely their own responsibility. All OSHA standards and rules must also be consulted and followed.
The hard copy print may only be distributed to other employees for their internal use within your organization. In addition, the electronic file may not be distributed elsewhere over computer networks or otherwise.
The Parallel Streak Method has proven effective over a number of years of use in providing evidence of aaycc activity against both Gram positive and Gram negative bacteria. Other organisms can also be used depending on the intended end-use. Check pH and adjust to 7. AATCC Test Method Limitations The method is not suitable for materials which are not migratory and are unable to diffuse thru merhod bacterial growth medium.
The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings.
AATCC Antibacterial Parallel Streak Test Method
Rectangular specimens cut 25 – 50 mm They can be any convenient size. The results of using this procedure have been demonstrated by Committee RA31 to be reproducible by various laboratories working with materials meyhod residual amounts of antibacterial agents as determined by 174 assay after multiple standard washings. A 50 TM Copyright? Many standard textile finishing chemicals, especially crease resistant and permanent press reagents, will often give strong antibacterial activity even after many washes.
BoxManassas VA ; tel: Allow agar to gel firmly before mthod. Some of the bacteria used in this test are pathogenic; i. This may be accomplished more easily by pressing the specimen to the agar surface with a biological section lifter or with a spatula which has been sterilized by flaming and then air cooled immediately before use.
AATCC Test Method 147
In the Parallel Streak Method, the agar surface is inoculated making it easier to distinguish between the test organism and contaminant organisms which may be present on the unsterilized specimen. AATCC MethodAntibacterial Finishes on Textile Materials, Assessment of, is a quantitative procedure which is adequately sensitive but is cumbersome and time consuming for routine quality control and screening tests. The test Method determines antibacterial activity of diffusable antimicrobial agents on treated textile materials.
AATCC grants you a license as follows: After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. After incubation, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. Neither the electronic file nor the hard copy print may be reproduced in any way.
If you feel that your comments have not received a fair hearing, you should make your views known to Christopher S. Notes and References Individual reprints single or multiple copies of this method may be obtained by contacting AATCC at the above address or tel: At the conclusion of two weeks, make a fresh transplant from stock culture. This is not necessary if prepared, dehydrated medium is used. Plug and sterilize at kPa 15 psi for 15 minutes. Therefore, when the intent is to demonstrate bacteriostatic activity by the diffusion of the antibacterial agent through agar, Method fulfills this need.
The criterion for passing the test must be agreed upon by the interested parties. Safety Precautions reasonable precaution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment. AATCC takes no position respecting the validity of any patent rights asserted in connection with any item mentioned in this test method.
AATCC Antimicrobial Testing Lab – Accugen Laboratories, Inc.
The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings. Take care not to break the surface of the agar while making the streaks. Culture Medium mm length permits the specimens to lie across 5 parallel inoculum streaks each of diminishing width methoc about 8 mm to 4 mm wide.
The precautions are ancillary to methdo testing procedures and are not intended to be all inclusive.
Treated materials should be compared to an untreated corresponding material and a material specimen with known bacteriostatic activity.
Antibacterial Activity Assessment of Textile Materials: